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Effect of electrical stimulation on β-adrenergic receptor population and cyclic AMP production in chicken and rat skeletal muscle cell cultures

  • Cellular Models
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Summary

Expression of the β-adrenergic receptor (βAR) and its coupling to cyclic AMP (cAMP) synthesis are important components of the signaling system that controls muscle atrophy and hypertrophy, and the goal of this study was to determine if electrical stimulation in a pattern simulating slow muscle contraction would alter the βAR response in primary cultures of avian and mammalian skeletal muscle cells. Specifically, chicken skeletal muscle cells and rat skeletal muscle cells that had been grown for 7d in culture were subjected to electrical stimulation for an additional 2 d at a pulse frequency of 0.5 pulses/sec and a pulse duration of 200 msec. In chicken skeletal muscle cells, the βAR population was not significantly affected by electrical stimulation; however, the ability of these cells to synthesize cyclic AMP was reduced by approximately one-half. In contrast, the βAR population in rat muscle cells was increased slightly but not significantly by electrical stimulation, and the ability of these cells to synthesize cyclic AMP was increased by almost twofold. The basal levels of intracellular cyclic AMP in neither rat muscle cells nor chicken muscle cells were affected by electrical stimulation.

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Author notes

  1. Catherine J. Strietzel

    Present address: Pfizer Central Research Division, Animal Health Biological Discovery, Mail Stop 8003, Eastern Point Road, 06340-8003, Groton, Connecticut

Authors and Affiliations

  1. Marshall Space Flight Center, SD48, Building 4481, 35812, Huntsville, Alabama

    Ronald B. Young & Kristin Y. Bridge

  2. Department of Biological Sciences, University of Alabama, 35899, Huntsville, Alabama

    Catherine J. Strietzel

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  1. Ronald B. Young
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  2. Kristin Y. Bridge
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  3. Catherine J. Strietzel
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Young, R.B., Bridge, K.Y. & Strietzel, C.J. Effect of electrical stimulation on β-adrenergic receptor population and cyclic AMP production in chicken and rat skeletal muscle cell cultures. In Vitro Cell.Dev.Biol.-Animal 36, 167–173 (2000). https://doi.org/10.1290/1071-2690(2000)036<0167:EOESOA>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1071-2690(2000)036<0167:EOESOA>2.0.CO;2

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